Calcium distribution within human erythrocytes.

Calcium distribution within human erythrocytes during endocytosis.

In order to study 45Ca movements within erythrocytes, a method was devised that had minimal deleterious effect on the treated erythrocytes. Agents that induce endocytosis in intact erythrocytes (primaquine, vinblastine, and chlorpromazine) caused a prompt movement of 45Ca from cytosol to membrane-associated sites. This drug-induced movement of 45Ca to membrane sites was blocked by depleting ery...

Expression and Distribution of Calcium-Binding Protein S100P in Human Placenta during Pregnancy

Polyphosphoinositide localization in human erythrocyte membranes

The asymmetrical distribution of the major phospholipids in biological membranes is now well established (Op den Kamp, 1979; Etemadi, 1980; van Deenen, 1981). In erythrocytes, the choline-containing phosphatidylcholine (PtdCho) and sphingomyelin are mainly located within the outer half of the membrane bilayer and the amino-containing phosphatidylethanolamine and phosphatidylserine within the in...

Erythrocyte-neutrophil interactions: formation of leukotriene B4 by transcellular biosynthesis.

Studies on the mechanism of leukotriene B4 biosynthesis in suspensions composed of neutrophils plus erythrocytes indicate that human erythrocytes convert neutrophil-derived leukotriene A4 into leukotriene B4. Leukotriene B4 formation by neutrophils in the presence of erythrocytes exceeded that from corresponding suspensions of neutrophils alone. The increase was proportional to the erythrocyte ...

Ca2+-activated K+ efflux limits complement-mediated lysis of human erythrocytes.

The lytic effect of complement on human erythrocytes has been reported by others to increase when Na+ is substituted for K+ in the external medium. In this paper we have investigated the hypothesis that net loss of K+ through a K+ transport pathway protects erythrocytes from complement-induced colloidosmotic swelling and lysis. Antibody-sensitized human erythrocytes containing different intrace...